CCL23 antibody (Biotin)

Catalog No. ABIN116287

This Rabbit Polyclonal antibody specifically detects CCL23 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for CCL23 antibody (Biotin) (ABIN116287)

Target: CCL23 (Chemokine (C-C Motif) Ligand 23 (CCL23))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL23 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL23 Antibody (Biotin)

Specificity: The antibody reacts with Macrophage Inflammatory Protein-3.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human MIP-3.

Application Details

Application Notes: ELISA: To detect hMIP-3 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.25 - 1.0 μg/mL. Used in conjunction with compatible secondaryreagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3. To detect hMIP-3 by sandwich ELISA (using 100 l/well antibody solution) a concentrationof 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, inconjuction with anti-human MIP-3 (cat-no. PP1089P1/2) as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant human MIP-3. Western Blot: To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hMIP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge the vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from date of despatch. Reconstituted antibody is stable for 2 weeks at 2-8 °C and for six months when stored in working aliquots at -20 °C.

Expiry Date: 12 months

Target Details for CCL23

Target: CCL23 (Chemokine (C-C Motif) Ligand 23 (CCL23))

Alternative Name: MIP3 / CCL23

Background: Macrophage Inflammatory Protein 3 (MIP3) is chemotactic for monocytes, resting T-lymphocytes, and neutrophils. It inhibits proliferation of myeloid progenitor cells and is highly expressed in adult lung, liver, skeletal muscle and pancreas. There are moderate levels of MIP3 in fetal liver, adult bone marrow and placenta. MIP3 belongs to the intercrine beta family (small cytokine c-c) (chemokine c-c).Synonyms: C-C motif chemokine 23, CCL-23, CK-beta-8, CKB-8, MIP-3, MPIF1, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, SCYA23, Small-inducible cytokine A23

Gene ID: 6368

NCBI Accession: NP_005055

UniProt: P55773