CNTF antibody (Biotin)

Catalog No. ABIN116299

This Rabbit Polyclonal antibody specifically detects CNTF in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for CNTF antibody (Biotin) (ABIN116299)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTF antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CNTF Antibody (Biotin)

Specificity: Ciliary Neurotrophic Factor (CNTF)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hCNTF (human CTNF).

Application Details

Application Notes: ELISA: To detect hCNTF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hCNTF. Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hCNTF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C or at -20 °C for longer.

Target Details for CNTF

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: CNTF

Background: Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.Synonyms: Ciliary Neurotrophic Factor

Gene ID: 1270

NCBI Accession: NP_000605

UniProt: P26441

Pathways: JAK-STAT Signaling