Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

GM-CSF antibody

This Rabbit Polyclonal antibody specifically detects GM-CSF in WB, EIA, IHC (p) and Func. It exhibits reactivity toward Human.
Catalog No. ABIN116305

Quick Overview for GM-CSF antibody (ABIN116305)

Target

See all GM-CSF (CSF2) Antibodies
GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity

  • 123
  • 47
  • 24
  • 11
  • 11
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
Human

Host

  • 106
  • 35
  • 33
  • 4
  • 1
Rabbit

Clonality

  • 112
  • 67
Polyclonal

Conjugate

  • 101
  • 23
  • 9
  • 6
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This GM-CSF antibody is un-conjugated

Application

  • 133
  • 77
  • 42
  • 32
  • 30
  • 29
  • 26
  • 20
  • 15
  • 13
  • 9
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
  • Specificity

    This antibody recognizes Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF). Other species not tested.

    Purification

    Affinity Chromatography employing immobilized hGM-CSF matrix

    Immunogen

    Highly pure (>98%) E.coli derived recombinant Human GM-CSF (Human Granulocyte Macrophage Colony Stimulating Factor)
  • Application Notes

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGMCSF (1.0 ng/mL), a concentration of 0.30-0.50 μg/mL of this antibody is required. ELISA: To detect hGM CSF by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hGM CSF. Western Blot: To detect hGM CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGM CSF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    Buffer

    PBS, pH 7.2 without preservatives

    Preservative

    Without preservative

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Target

    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

    Alternative Name

    GM-CSF

    Background

    The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

    Gene ID

    1437

    NCBI Accession

    NP_000749

    UniProt

    P04141

    Pathways

    JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
You are here:
Chat with us!