VEGFA antibody (Biotin)

Catalog No. ABIN116331

The Biotin-conjugated Rabbit Polyclonal anti-VEGFA antibody (ABIN116331) specifically detects VEGFA in WB and EIA. The antibody is reactive with Mouse samples.

Quick Overview for VEGFA antibody (Biotin) (ABIN116331)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-VEGFA Antibody (Biotin)

Specificity: Reacts with Vascular Endothelial Growth Factor (VEGF).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mVEGF (murine VEGF)

Application Details

Application Notes: ELISA: To detect mVEGF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A

Background: Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gene ID: 22339

NCBI Accession: NP_001020421

UniProt: Q00731

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling