TWEAK antibody

Catalog No. ABIN116357

The Goat Polyclonal anti-TWEAK antibody has been validated for WB, EIA and Func. It is suitable to detect TWEAK in samples from Human.

Quick Overview for TWEAK antibody (ABIN116357)

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TWEAK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-TWEAK Antibody

Specificity: Human TWEAK

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTWEAK.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTWEAK (200 ng/mL), a concentration of 0.1 - 0.2 μg/mL of this antibody is required. ELISA: To detect hTWEAK by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTWEAK is 1.5-3.0 ng/lane, under either reducing or ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for TWEAK

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: TWEAK / TNFSF12

Background: Synonyms: APO3 ligand, APO3L, DR3LG, TNF-related weak inducer of apoptosis, Tumor necrosis factor ligand superfamily member 12

Gene ID: 8742

NCBI Accession: NP_003800

UniProt: O43508

Pathways: Apoptosis