TNFSF14 antibody

Catalog No. ABIN116433

The Rabbit Polyclonal anti-TNFSF14 antibody (ABIN116433) specifically detects TNFSF14 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for TNFSF14 antibody (ABIN116433)

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFSF14 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TNFSF14 Antibody

Purification: Affinity chromatography employing immobilized hLIGHT matrix.

Immunogen: Highly pure (>98%) recombinant hLIGHT

Application Details

Application Notes: Neutralization. ELISA: To detect hLIGHT by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hLIGHT. Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLIGHT is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: 0.5 X PBS pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for TNFSF14

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Alternative Name: CD258 / LIGHT

Background: LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors, the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).Synonyms: HVEM-L, HVEML, Herpesvirus entry mediator-ligand, TNFSF14, Tumor necrosis factor ligand superfamily member 14

Gene ID: 8740

NCBI Accession: NP_003798

UniProt: O43557

Pathways: Cancer Immune Checkpoints