KIT Ligand antibody

Catalog No. ABIN116453

The Rabbit Polyclonal anti-KIT Ligand antibody is suitable to detect KIT Ligand in samples from Rat. It has been validated for WB, EIA and Neut.

Quick Overview for KIT Ligand antibody (ABIN116453)

Target: KIT Ligand (KITLG)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)

Product Details anti-KIT Ligand Antibody

Specificity: Recognizes Rat SCF

Purification: Affinity Chromatography employing an immobilized Rat SCF matrix.

Immunogen: Highly pure (>98%) recombinant Rat SCF.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of ratSCF(15 ng/mL), a concentration of 0.08-0.12 μg/mL of this antibody is required. ELISA: To detect rat SCF by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant rat SCF. Western Blot: To detect rat SCF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat SCF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF

Background: Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gene ID: 60427

NCBI Accession: NP_068615

UniProt: P21581

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway