BMP2 antibody (Biotin)

Catalog No. ABIN116463

This anti-BMP2 antibody is a Rabbit Polyclonal antibody detecting BMP2 in WB and EIA. Suitable for Human.

Quick Overview for BMP2 antibody (Biotin) (ABIN116463)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BMP2 Antibody (Biotin)

Specificity: The antibody recognizes Human BMP2.Other species not tested.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human BMP-2.

Application Details

Application Notes: ELISA: To detect hBMP-2 by direct ELISA (using 100 l/well antibody solution) aconcentration of 0.15 - 0.30 μg/mL of this antibody is required. This antibody inconjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hBMP-2. Western Blot: To detect hBMP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBMP-2 is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C. For prolonged storage of reconstituted antibody solution we strongly recommend addition of a stabilizing protein (e.g. 0.5% BSA) and a preservative (e.g. Thimerosal or Sodium Azide).

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP2 / BMP2A

Background: BMP2 belongs to the transforming growth factor beta (TGFB) superfamily of secreted growth factors. It is a disulfide linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.Synonyms: BMP-2, BMP-2A, Bone morphogenetic protein 2

Gene ID: 650

NCBI Accession: NP_001191

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation