LGALS1/Galectin 1 antibody

Catalog No. ABIN116481

This anti-LGALS1/Galectin 1 antibody is a Rabbit Polyclonal antibody detecting LGALS1/Galectin 1 in WB and EIA. Suitable for Human.

Quick Overview for LGALS1/Galectin 1 antibody (ABIN116481)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGALS1/Galectin 1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-LGALS1/Galectin 1 Antibody

Specificity: Recognizes Galectin-1

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography employing immobilized Human Galectin-1 matrix.

Immunogen: E. Coli derived Recombinant Human Galectin-1.

Application Details

Application Notes: Sandwich ELISA: To detect Human Galectin-1 (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human Galectin-1 (PP1223B1 and PP1223B2)as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantHuman Galectin-1. Western Blot: To detect Human Galectin-1 by this analysis, the antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGalectin-1 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin-1

Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.Synonyms: 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Galectin1, HBL, HPL, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, MAPK-activating protein PM12, S-Lac lectin 1

Molecular Weight: approx. 150 kDa

Gene ID: 3956

NCBI Accession: NP_002296

UniProt: P09382

Pathways: Carbohydrate Homeostasis