GDF3 antibody

Catalog No. ABIN116497

This Rabbit Polyclonal antibody specifically detects GDF3 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for GDF3 antibody (ABIN116497)

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDF3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GDF3 Antibody

Specificity: This antibody is specific to GDF3.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant hGDF-3 (human GDF-3)

Application Details

Application Notes: ELISAIndirect: To detect hGDF-3 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hGDF-3. Sandwich: To detect hGDF-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human GDF-3 (PP1227B1, PP1227B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGDF-3. Western blotTo detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hGDF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for GDF3

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Alternative Name: GDF3

Background: GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.Synonyms: GDF-3, Growth/differentiation factor 3

Gene ID: 9573

NCBI Accession: NP_065685

UniProt: Q9NR23