D Amino Acid Oxidase antibody

Catalog No. ABIN116616

The Sheep Polyclonal anti-D Amino Acid Oxidase antibody (ABIN116616) specifically detects D Amino Acid Oxidase in WB, IP and EIA. The antibody is reactive with Pig samples.

Quick Overview for D Amino Acid Oxidase antibody (ABIN116616)

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This D Amino Acid Oxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-D Amino Acid Oxidase Antibody

Specificity: This antibody detects porcine D-amino-acid oxidase. Immunoelectrophoresis give a single precipitin arc against purified and partially purified D-amino-acid oxidase [pig kidney].

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: D-Amino Acid Oxidase [Pig Kidney].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of D-Amino AcidOxidase [Pig Kidney] in a standard sandwich ELISA using Peroxidase conjugated affinitypurified anti-Sheep IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 20,000 to 1: 100,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, with 0.01 % Sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.

Target Details for D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Alternative Name: D-Amino-Acid Oxidase

Background: This gene encodes the peroxisomal enzyme D-amino-acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino-acids, but it is inactive on the naturally occurring L amino acids. Its biological function is not known, it has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino-acids accumulated during aging, or it may be a fossil enzyme without a current function.Synonyms: DAAO, DAMOX, DAO, DAO1, OXDA

Gene ID: 1610

NCBI Accession: NP_001908

UniProt: P14920