GLB1 antibody

Catalog No. ABIN116640

The Rabbit Polyclonal anti-GLB1 antibody (ABIN116640) specifically detects GLB1 in WB, IHC (p), EIA and IHC (fro). The antibody is reactive with E. coli samples.

Quick Overview for GLB1 antibody (ABIN116640)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GLB1 Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E. coli]. Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined.

Purification: Purified from monospecific antiserum by Delipidation and Defribination.

Immunogen: Beta Galactosidase [E. coli].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/65,000. Western blot: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80 mg/mL (by refractometry)

Buffer: 0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % Sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: beta Galactosidase (GAL)

Background: Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag

Gene ID: 945006

NCBI Accession: AP_000996

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process