Catalase antibody

Catalog No. ABIN116677

This Rabbit Polyclonal antibody specifically detects Catalase in WB, IP and EIA. It exhibits reactivity toward Cow.

Quick Overview for Catalase antibody (ABIN116677)

Target: Catalase (CAT)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Catalase Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Catalase from Bovine liver.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Catalase [Bovineliver] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (R1364HRP) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 26,000 to 1: 142,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 5.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing no preservative.

Preservative: Without preservative

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: Catalase

Background: Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.Synonyms: CAT, Peroxisome Marker

Gene ID: 531682

NCBI Accession: NP_001030463

UniProt: P00432

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism