CES1 antibody

Catalog No. ABIN116701

This Rabbit Polyclonal antibody specifically detects CES1 in WB and EIA. It exhibits reactivity toward Pig.

Quick Overview for CES1 antibody (ABIN116701)

Target: CES1 (Carboxylesterase 1 (CES1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CES1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CES1 Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Esterase [Porcine Liver]. Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Esterase [Porcine Liver].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Esterase[Porcine Liver] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 8,000 to1: 40,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative., None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. For extended storage aliquot contents and freeze at or below -20 °C. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Alternative Name: CES1 / ACAT

Background: Synonyms: Acyl coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, CES2, Egasyn, HMSE, Liver carboxylesterase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, SES1, Serine esterase 1, Triacylglycerol hydrolase, hBr1

Gene ID: 397478

NCBI Accession: NP_999411

UniProt: Q29550

Pathways: Monocarboxylic Acid Catabolic Process