Glucose Oxidase antibody

Catalog No. ABIN116717

The Rabbit Polyclonal anti-Glucose Oxidase antibody is suitable to detect Glucose Oxidase in samples from Aspergillus. It has been validated for WB, IP and EIA.

Quick Overview for Glucose Oxidase antibody (ABIN116717)

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Glucose Oxidase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Glucose Oxidase [Aspergillus niger].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Glucose Oxidase[Aspergillus niger] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 16,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium phosphate, 0.15 M Sodium chloride, pH 7.2 containing no preservatives/stabilizers

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase

Background: Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.Synonyms: Beta-D-glucose:oxygen 1-oxido-reductase, Glucose oxyhydrase, gox

Gene ID: 5061

UniProt: P13006