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GLUD1 antibody

GLUD1 Reactivity: Cow WB, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN116725
  • Target See all GLUD1 Antibodies
    GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
    Reactivity
    • 38
    • 16
    • 15
    • 14
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow
    Host
    • 41
    • 9
    • 1
    Rabbit
    Clonality
    • 41
    • 10
    Polyclonal
    Conjugate
    • 30
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GLUD1 antibody is un-conjugated
    Application
    • 31
    • 21
    • 18
    • 11
    • 9
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
    Purification
    Delipidation and defibrination.
    Immunogen
    A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
    Top Product
    Discover our top product GLUD1 Primary Antibody
  • Application Notes
    This antibody has been tested for use in ELISA and by Western blot. Bovine glutamatedehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect aband approximately 62 kDa in size corresponding to glutamate dehydrogenase monomersubunit by Western blotting in the appropriate cell or tissue extract. Recommended Dilutions: ELISA: 1/4,000-1/16,000Western blot: 1/1,000-1/3,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore with 2.0 mL of deionized water (or equivalent).
    Concentration
    85.0 mg/mL (by refractometry)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
  • Target
    GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
    Alternative Name
    Glutamate Dehydrogenase 1 (GLUD1 Products)
    Background
    Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1, EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.Synonyms: GDH1, GLUD, GLUD1
    Gene ID
    2746
    NCBI Accession
    NP_005262
    UniProt
    P00367
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Warburg Effect
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