GLUD1 antibody

Catalog No. ABIN116725

Product Details anti-GLUD1 Antibody

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Purification: Delipidation and defibrination.

Immunogen: A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.

Application Details

Application Notes: This antibody has been tested for use in ELISA and by Western blot. Bovine glutamatedehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect aband approximately 62 kDa in size corresponding to glutamate dehydrogenase monomersubunit by Western blotting in the appropriate cell or tissue extract. Recommended Dilutions: ELISA: 1/4,000-1/16,000Western blot: 1/1,000-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: Glutamate Dehydrogenase 1 (GLUD1 Products)

Background: Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1, EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.Synonyms: GDH1, GLUD, GLUD1

Gene ID: 2746

NCBI Accession: NP_005262

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect