Hexokinase 1 antibody

Catalog No. ABIN116752

This Rabbit Polyclonal antibody specifically detects Hexokinase 1 in WB and EIA. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for Hexokinase 1 antibody (ABIN116752)

Target: Hexokinase 1 (HK1)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Hexokinase 1 Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Hexokinase [Yeast].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Hexokinase[Yeast] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 10,000 to1: 40,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: Hexokinase-1

Background: Synonyms: Brain form hexokinase, HK1, Hexokinase type I

Gene ID: 2542634

NCBI Accession: NP_592948

UniProt: Q09756

Pathways: Carbohydrate Homeostasis, Warburg Effect