Hexokinase 1 antibody (N-Term)

Catalog No. ABIN317095

This Mouse Monoclonal antibody specifically detects Hexokinase 1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for Hexokinase 1 antibody (N-Term) (ABIN317095)

Target: Hexokinase 1 (HK1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 4D7

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: AA 1-917, N-Term

Specificity: The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse (see pictures below).

Characteristics: Synonyms: HK1, Hexokinase type I, Brain form hexokinase

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Recombinant Human Hexokinase 1 (1-917 aa) purified from E. coli

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blot (1: 1,000). Immunofluorescence (1: 1,000). Immunohistochemistry on Paraffin Sections (5 μg/mL): This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: Hexokinase-1

Background: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.Synonyms: Brain form hexokinase, HK1, Hexokinase type I

Gene ID: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect