Pancreatic Islet Lysate antibody

Catalog No. ABIN116793

The Dog Polyclonal anti-Pancreatic Islet Lysate antibody (ABIN116793) specifically detects Pancreatic Islet Lysate in WB and EIA. The antibody is reactive with Pig samples.

Quick Overview for Pancreatic Islet Lysate antibody (ABIN116793)

Target: Pancreatic Islet Lysate

Reactivity: Pig

Host: Dog

Clonality: Polyclonal

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details

Purification: Multi-step process.

Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 μg of Pancreatic IsletLysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. Aworking dilution of 1: 50 to 1: 100 is suggested for this product. This product has beenassayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane byImmunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer tonitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG[H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1: 50 to1: 200 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: -20 °C

Storage Comment: Store vial at -70 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Target Details

Target: Pancreatic Islet Lysate