FLIP antibody (AA 1-480)

Catalog No. ABIN1169019

Cited in 7+ publications. The Rat Monoclonal anti-FLIP antibody (Clone Dave-2) (ABIN1169019) specifically detects FLIP in WB and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for FLIP antibody (AA 1-480) (ABIN1169019)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: Dave-2

Product Details anti-FLIP Antibody

Binding Specificity: AA 1-480

Purpose: anti-FLIP, mAb (Dave-2)

Specificity: Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP.

Characteristics: Monoclonal Antibody. Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP. Isotype: Rat IgG2a. Clone: Dave-2. Applications: IP, WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.

Immunogen: Recombinant human FLIP (aa 1-480).

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-FLIP antibody (ABIN1169019)

Van Opdenbosch, Van Gorp, Verdonckt, Saavedra, de Vasconcelos, Gonçalves, Vande Walle, Demon, Matusiak, Van Hauwermeiren, DHont, Hochepied, Krautwald, Kanneganti, Lamkanfi: "Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC4." in: Cell reports, Vol. 21, Issue 12, pp. 3427-3444, (2018) (PubMed).

Dannappel, Vlantis, Kumari, Polykratis, Kim, Wachsmuth, Eftychi, Lin, Corona, Hermance, Zelic, Kirsch, Basic, Bleich, Kelliher, Pasparakis: "RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis." in: Nature, Vol. 513, Issue 7516, pp. 90-4, (2014) (PubMed).

Lens, Kataoka, Fortner, Tinel, Ferrero, MacDonald, Hahne, Beermann, Attinger, Orbea, Budd, Tschopp: "The caspase 8 inhibitor c-FLIP(L) modulates T-cell receptor-induced proliferation but not activation-induced cell death of lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5419-33, (2002) (PubMed).

Gómez-Angelats, Cidlowski: "Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 44944-52, (2001) (PubMed).

Micheau, Lens, Gaide, Alevizopoulos, Tschopp: "NF-kappaB signals induce the expression of c-FLIP." in: Molecular and cellular biology, Vol. 21, Issue 16, pp. 5299-305, (2001) (PubMed).

Djerbi, Darreh-Shori, Zhivotovsky, Grandien: "Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms." in: Scandinavian journal of immunology, Vol. 54, Issue 1-2, pp. 180-9, (2001) (PubMed).

Rescigno, Piguet, Valzasina, Lens, Zubler, French, Kindler, Tschopp, Ricciardi-Castagnoli et al.: "Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate ..." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1661-8, (2000) (PubMed).

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: FLIP

Background:

Alternate Names/Synonyms: I-FLICE, CLARP, CASPER, Usurpin, CASH, FLAME-1

Product Description: FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development