NAMPT antibody

Catalog No. ABIN1169025

Product Details anti-NAMPT Antibody

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAMPT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: Recognizes human Nampt. Detects a band of ~54 kDa by Western blot.

Cross-Reactivity: Human

Cross-Reactivity (Details): Weakly cross-reacts with mouse Nampt.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human Nampt (visfatin/PBEF).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

References for anti-NAMPT antibody (ABIN1169025)

Moschen, Kaser, Enrich, Mosheimer, Theurl, Niederegger, Tilg: "Visfatin, an adipocytokine with proinflammatory and immunomodulating properties." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 3, pp. 1748-58, (2007) (PubMed).

Nowell, Richards, Fielding, Ognjanovic, Topley, Williams, Bryant-Greenwood, Jones: "Regulation of pre-B cell colony-enhancing factor by STAT-3-dependent interleukin-6 trans-signaling: implications in the pathogenesis of rheumatoid arthritis." in: Arthritis and rheumatism, Vol. 54, Issue 7, pp. 2084-95, (2006) (PubMed).

Bae, Kim, Kim, Kim, Koo, Jang, Yun, Yoo, Bae: "Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1." in: FEBS letters, Vol. 580, Issue 17, pp. 4105-13, (2006) (PubMed).

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Nampt (Visfatin/PBEF) (NAMPT Products)

Background: Nampt also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

UniProt: P43490