NAMPT antibody (Biotin)

Catalog No. ABIN1169027

The Rabbit Polyclonal anti-NAMPT antibody has been validated for WB, ELISA and IHC. It is suitable to detect NAMPT in samples from Human.

Quick Overview for NAMPT antibody (Biotin) (ABIN1169027)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAMPT antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NAMPT Antibody (Biotin)

Specificity: Recognizes human Nampt. Detects a band of ~54 kDa by Western blot.

Cross-Reactivity: Human

Cross-Reactivity (Details): Weakly cross-reacts with mouse Nampt.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human Nampt (visfatin/PBEF).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Nampt (Visfatin/PBEF)

Background: Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

UniProt: P43490