N-Acetyl-D-Mannosamine Dehydrogenase antibody

Catalog No. ABIN116910

The Goat Polyclonal anti-N-Acetyl-D-Mannosamine Dehydrogenase antibody (ABIN116910) specifically detects N-Acetyl-D-Mannosamine Dehydrogenase in EIA, IP and WB. The antibody is reactive with E. coli samples.

Quick Overview for N-Acetyl-D-Mannosamine Dehydrogenase antibody (ABIN116910)

Target: N-Acetyl-D-Mannosamine Dehydrogenase

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Application: Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Key Features:

• NLRP3 antibody for reliable recognition of mouse and human NLRP3/NALP3
• This un-conjugated monoclonal antibody has 60 PubMed citations

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant]. Cross reactivity against N-Acyl Mannosamine-1-Dehydrogenase from other sources is unknown.

Purification: Multistep process.

Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions. This product has been assayed against 1.0 μg of N-AcylMannosamine-1-Dehydrogenase [E. coli Recombinant] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product:
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent)

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details

Target: N-Acetyl-D-Mannosamine Dehydrogenase

Background: N acylmannosamine 1 dehydrogenase belongs to the short chain dehydrogenases/ reductases family (SDR). It acts on acetyl D mannosamine and glycolyl D mannosamine.Synonyms: JW5599, b3787, rffD, wecC

Gene ID: 948977

NCBI Accession: AP_004010

UniProt: P27829