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eGFP antibody (Enhanced Green Fluorescent Protein) Primary Antibody

eGFP Reactivity: Aequorea victoria ICC, IP, ELISA Host: Human Monoclonal G3 unconjugated
Pubmed (1 reference)
Catalog No. ABIN1169226
$474.38
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 5 to 8 Business Days
  • Target See all eGFP products
    eGFP
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    Aequorea victoria
    Host
    • 5
    • 5
    • 1
    • 1
    Human
    Clonality
    • 6
    • 6
    Monoclonal
    Conjugate
    • 10
    • 1
    • 1
    This eGFP antibody is un-conjugated
    Application
    • 9
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA
    Specificity
    Recognizes EGFP, Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP).
    Purification
    Purified from HEK 293 cell culture supernatant.
    Purity
    >95 % (SDS-PAGE)
    Immunogen
    Enhanced Green Fluorescent Proteins (EGFP).
    Clone
    G3
    Isotype
    IgG2
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    anti-EGFP, monoclonal antibody (recombinant) (G3) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    Expiry Date
    12 months
  • Moutel, Vielemeyer, Jin, Divoux, Benaroch, Perez: "Fully in vitro selection of recombinant antibodies." in: Biotechnology journal, Vol. 4, Issue 1, pp. 38-43, (2009) (PubMed).

  • Target
    eGFP
    Alternative Name
    EGFP (eGFP Products)
    Background
    Anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
    UniProt
    P42212
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