Enhanced Green Fluorescent Protein (eGFP) antibody Primary Antibody
eGFP
Reactivity: Aequorea victoria
ICC, IP, ELISA
Host: Human
Monoclonal
G3
Catalog No. ABIN1169226
$517.50
Plus shipping costs $45.00
100 μg
local_shipping
Shipping to:
United States
Delivery in 5 to 8 Business Days
-
- Target
- Antibody Type
- Recombinant Antibody
- Reactivity
- Aequorea victoria
- Host
- Human
- Clonality
- Monoclonal
- Conjugate
- Un-conjugated
- Application
- Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA
- Specificity
- Recognizes EGFP, Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP).
- Purification
- Purified from HEK 293 cell culture supernatant.
- Purity
- >95 % (SDS-PAGE)
- Immunogen
- Enhanced Green Fluorescent Proteins (EGFP).
- Clone
- G3
- Isotype
- IgG2
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
anti-EGFP, monoclonal antibody (recombinant) (G3) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C. - Expiry Date
- 12 months
-
-
Moutel, Vielemeyer, Jin, Divoux, Benaroch, Perez: "Fully in vitro selection of recombinant antibodies." in: Biotechnology journal, Vol. 4, Issue 1, pp. 38-43, 2009 (PubMed).
-
Moutel, Vielemeyer, Jin, Divoux, Benaroch, Perez: "Fully in vitro selection of recombinant antibodies." in: Biotechnology journal, Vol. 4, Issue 1, pp. 38-43, 2009 (PubMed).
-
- Target
- Alternative Name
- EGFP
- Background
- Anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
- UniProt
- P42212
You are here: