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Enhanced Green Fluorescent Protein (eGFP) antibody Primary Antibody

eGFP Reactivity: Aequorea victoria ICC, IP, ELISA Host: Human Monoclonal G3
Pubmed (1)
Catalog No. ABIN1169226
$517.50
Plus shipping costs $45.00
100 μg
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  • Target
    Antibody Type
    Recombinant Antibody
    Reactivity
    Aequorea victoria
    Host
    Human
    Clonality
    Monoclonal
    Conjugate
    Un-conjugated
    Application
    Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA
    Specificity
    Recognizes EGFP, Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP).
    Purification
    Purified from HEK 293 cell culture supernatant.
    Purity
    >95 % (SDS-PAGE)
    Immunogen
    Enhanced Green Fluorescent Proteins (EGFP).
    Clone
    G3
    Isotype
    IgG2
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    anti-EGFP, monoclonal antibody (recombinant) (G3) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    Expiry Date
    12 months
  • Moutel, Vielemeyer, Jin, Divoux, Benaroch, Perez: "Fully in vitro selection of recombinant antibodies." in: Biotechnology journal, Vol. 4, Issue 1, pp. 38-43, 2009 (PubMed).

  • Target
    Alternative Name
    EGFP
    Background
    Anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
    UniProt
    P42212
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