NAIP1/2/5 (AA 159-227), (BIR2 Domain) antibody

Catalog No. ABIN1169396

The Mouse Monoclonal anti-NAIP1/2/5 antibody is suitable to detect NAIP1/2/5 in samples from Mouse. It has been validated for WB.

Quick Overview for NAIP1/2/5 (AA 159-227), (BIR2 Domain) antibody (ABIN1169396)

Target: NAIP1/2/5

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB)

Clone: Naipa-1

Product Details

Binding Specificity: AA 159-227, BIR2 Domain

Purpose: anti-NAIP1/2/5 (mouse), mAb (Naipa-1)

Specificity: Recognizes mouse NAIP1, 2 and 5. Not tested on mouse NAIP6 and mouse NAIP7 but should also work due to high homology in the BIR2 domain.

Characteristics: Monoclonal Antibody. Recognizes mouse NAIP1, 2 and 5. Not tested on mouse NAIP6 and mouse NAIP7 but should also work due to high homology in the BIR2 domain. Isotype: Mouse IgG2bkappa. Clone: Naipa-1. Applications: WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor (NLR) family are critical components of the inflammasome that link danger-signals to caspase-1 activation. The NLR member IPAF/NLRC4 senses bacterial flagellin and a conserved type III secretion system (TTSS) rod component. A member of the NLR family, NAIP5 directly and specifically interacts with flagellin, triggers a physical NAIP5-NLRC4 association leading to NLRC4 activation. The related NAIP2 functions analogously to NAIP5, serving as a specific inflammasome receptor for TTSS rod proteins such as Salmonella PrgJ and Burkholderia BsaK.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse NAIP5 (BIR2 domain/aa 159-227).

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details

Target: NAIP1/2/5

Background:

Alternate Names/Synonyms: Neuronal Apoptosis Inhibitory Protein 1, 2, 5, Baculoviral IAP Repeat-containing Protein 1a, 1b, 1e, Birc 1a, 1b, 1e

Product Description: The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor (NLR) family are critical components of the inflammasome that link danger-signals to caspase-1 activation. The NLR member IPAF/NLRC4 senses bacterial flagellin and a conserved type III secretion system (TTSS) rod component. A member of the NLR family, NAIP5 directly and specifically interacts with flagellin, triggers a physical NAIP5-NLRC4 association leading to NLRC4 activation. The related NAIP2 functions analogously to NAIP5, serving as a specific inflammasome receptor for TTSS rod proteins such as Salmonella PrgJ and Burkholderia BsaK.

UniProt: Q9R016