ACE2 antibody

Catalog No. ABIN1169449

This Mouse Monoclonal antibody specifically detects ACE2 in WB, ELISA and FACS. It exhibits reactivity toward Human. It has been mentioned in 4+ publications

Quick Overview for ACE2 antibody (ABIN1169449)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: AC18F

Product Details anti-ACE2 Antibody

Purpose: anti-ACE2 (human), mAb (AC18F)

Specificity: Recognizes human ACE2. Does not detect recombinant human ACE2 that has a tag at the C-terminus of the protein.

Characteristics: Monoclonal Antibody. Recognizes human ACE2. Isotype: Mouse IgG1kappa. Clone: AC18F. Applications: ELISA, FACS, WB. Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. ACE2 has direct effects on cardiac functions. It is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE2 converts angiotensin I to angiotensin 1-9. ACE2 was also identified as a functional receptor for SARS coronavirus.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human ACE2.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.2μm-filtered solution in PBS, pH 7.4, 10 % glycerol.

Preservative: Without preservative

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-ACE2 antibody (ABIN1169449)

Chaouat, Achdout, Kol, Berhani, Roi, Vitner, Melamed, Politi, Zahavy, Brizic, Lenac Rovis, Alfi, Wolf, Jonjic, Israely, Mandelboim: "SARS-CoV-2 receptor binding domain fusion protein efficiently neutralizes virus infection." in: PLoS pathogens, Vol. 17, Issue 12, pp. e1010175, (2022) (PubMed).

Chaouat, Brizic, Kucan Brlic, Atari, Kliker, Alfi, Mandelboim, Wolf, Tafish, Kol, Jonjic, Mandelboim: "Anti-human ACE2 antibody neutralizes and inhibits virus production of SARS-CoV-2 variants of concern." in: iScience, Vol. 25, Issue 9, pp. 104935, (2022) (PubMed).

Buchrieser, Dufloo, Hubert, Monel, Planas, Rajah, Planchais, Porrot, Guivel-Benhassine, Van der Werf, Casartelli, Mouquet, Bruel, Schwartz: "Syncytia formation by SARS-CoV-2-infected cells." in: The EMBO journal, pp. e106267, (2020) (PubMed).

Zulli, Rai, Buxton, Burrell, Hare: "Co-localization of angiotensin-converting enzyme 2-, octomer-4- and CD34-positive cells in rabbit atherosclerotic plaques." in: Experimental physiology, Vol. 93, Issue 5, pp. 564-9, (2008) (PubMed).

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2

Background:

Alternate Names/Synonyms: SARS Receptor, Angiotensin-converting Enzyme 2, ACEH, Metalloprotease MPROT15, SARS-CoV-2 Receptor

Product Description: Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42 % sequence identity with ACE, and its cytoplasmic and transmembrane domains show 48 % homology to the protein collectrin that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19.

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour