Angiotensin II antibody (Biotin)

Catalog No. ABIN1171912

This Rabbit Polyclonal antibody specifically detects Angiotensin II in IHC, WB and ICC. It exhibits reactivity toward Human.

Quick Overview for Angiotensin II antibody (Biotin) (ABIN1171912)

Target: Angiotensin II (Ang II)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiotensin II antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-Angiotensin II Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Angiotensin II (AngII)

Specificity: The antibody is a rabbit polyclonal antibody raised against AngII. It has been selected for its ability to recognize AngII in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA005Hu01Polyclonal Antibody to Angiotensin II (AngII)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Angiotensin II

Target: Angiotensin II (Ang II)

Alternative Name: Angiotensin II

Background: Ang-II, Angiotensin-2

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity