HBEGF antibody (Biotin)

Catalog No. ABIN1174672

This Rabbit Polyclonal antibody specifically detects HBEGF in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for HBEGF antibody (Biotin) (ABIN1174672)

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBEGF antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-HBEGF Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)

Specificity: The antibody is a rabbit polyclonal antibody raised against HBEGF. It has been selected for its ability to recognize HBEGF in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: The antibody is a rabbit polyclonal antibody raised against HBEGF conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HBEGF

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Alternative Name: Heparin Binding Epidermal Growth Factor Like Growth Factor

Background: HB-EGF, DTR, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor, Proheparin-binding EGF-like growth factor, Diphtheria toxin receptor

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway