S100A9 antibody (AA 1-114) (Biotin)
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- Target See all S100A9 Antibodies
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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Binding Specificity
- AA 1-114
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This S100A9 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Sequence
- MGHHHHHHSG S-MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENKNEKVIE HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTP
- Specificity
- It has been selected for its ability to recognize S100A9 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant S100A9 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against S100A9 conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product S100A9 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant S100A9 (Met1~Pro114) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
- Abstract
- S100A9 Products
- Synonyms
- 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, S100A9 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody
- Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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