CAPN1 antibody (AA 75-356) (Biotin)

Catalog No. ABIN1175260

This Rabbit Polyclonal antibody specifically detects CAPN1 in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for CAPN1 antibody (AA 75-356) (Biotin) (ABIN1175260)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CAPN1 Antibody (Biotin)

Binding Specificity: AA 75-356

Purpose: Biotin-Linked Polyclonal Antibody to Calpain 1 (CAPN1)

Sequence: MGHHHHHHSG SEF-PNSSKT YGIKWKRPTE LLSNPQFIVD GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQNGYAGIFH FQLWQFGEWV DVVVDDLLPI KDGKLVFVHS AEGNEFWSAL LEKAYAKVNG SYEALSGGST SEGFEDFTGG VTEWYELRKA PSDLYQIILK ALERGSLLGC SIDISSVLDM EAITFKKLVK GHAYSVTGAK QVNYRGQVVS LIRMRNPWGE VEWTGAWSDS SSEWNNVDPY ERDQLRVKME DGEFWMSFRD FMR

Specificity: The antibody is a rabbit polyclonal antibody raised against CAPN1. It has been selected for its ability to recognize CAPN1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant CAPN1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CAPN1 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: Calpain 1

Background: PIG30, CANPL1, MuCANP, MuCL, Calpain 1, Large Subunit, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain