CAPN1 antibody (AA 75-356) (Biotin)

Catalog No. ABIN1175260

Product Details anti-CAPN1 Antibody (Biotin)

Target: CAPN1 (Calpain 1 (CAPN1))

Binding Specificity: AA 75-356

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequence: MGHHHHHHSG SEF-PNSSKT YGIKWKRPTE LLSNPQFIVD GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQNGYAGIFH FQLWQFGEWV DVVVDDLLPI KDGKLVFVHS AEGNEFWSAL LEKAYAKVNG SYEALSGGST SEGFEDFTGG VTEWYELRKA PSDLYQIILK ALERGSLLGC SIDISSVLDM EAITFKKLVK GHAYSVTGAK QVNYRGQVVS LIRMRNPWGE VEWTGAWSDS SSEWNNVDPY ERDQLRVKME DGEFWMSFRD FMR

Specificity: It has been selected for its ability to recognize CAPN1 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant CAPN1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CAPN1 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant CAPN1 (Pro75~Arg343) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Abstract: CAPN1 Products

Synonyms: CAPN1 antibody, Capa-1 antibody, Capa1 antibody, mu-calpin antibody, Capn1 antibody, fj67g01 antibody, wu:fj67g01 antibody, calpain 1 antibody, zgc:55262 antibody, CAPN1 antibody, Capn1 antibody, zgc:55262 antibody