BLMH antibody (AA 213-447) (Biotin)

Catalog No. ABIN1175600

Product Details anti-BLMH Antibody (Biotin)

Target: BLMH (Bleomycin Hydrolase (BLMH))

Binding Specificity: AA 213-447

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLMH antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS- MEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAW

Specificity: It has been selected for its ability to recognize BLMH in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant BLMH expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against BLMH conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant BLMH (Met213~Trp447) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for BLMH

Target: BLMH (Bleomycin Hydrolase (BLMH))

Abstract: BLMH Products

Synonyms: AI035728 antibody, Bh antibody, Bmh antibody, wu:fb13c01 antibody, zgc:66261 antibody, bh antibody, bmh antibody, BH antibody, BMH antibody, bleomycin hydrolase antibody, Bleomycin hydrolase antibody, bleomycin hydrolase S homeolog antibody, BLMH antibody, pepC antibody, LOC5569945 antibody, Apre_0140 antibody, Apar_0899 antibody, Smon_1289 antibody, Kfla_1242 antibody, Snas_3912 antibody, BLJ_1941 antibody, Olsu_1085 antibody, PGTDC60_0106 antibody, Blmh antibody, blmh antibody, blmh.S antibody