SFRP5 antibody (AA 45-162) (Biotin)

Catalog No. ABIN1175833

Product Details anti-SFRP5 Antibody (Biotin)

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Binding Specificity: AA 45-162

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFRP5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequence: MGHHHHHHSG SEF-SKPPQC LDIPADLPLC HTVGYKRMRL PNLLEHESLA EVKQQASSWL PLLAKRCHSD TQVFLCSLFA PVCLDRPIYP CRSLCEAVRA GCAPLMEAYG FPWPEMLHCH KFPLDNDLCI AV

Specificity: It has been selected for its ability to recognize SFRP5 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant SFRP5 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against SFRP5 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant SFRP5 (Ser45~Val162) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for SFRP5

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Alternative Name: Secreted Frizzled Related Protein 5 (SFRP5) (SFRP5 Products)

Synonyms: SARP3 antibody, AI605071 antibody, D144 antibody, frp1 antibody, zgc:101849 antibody, Sfrp5 antibody, XsFRP5 antibody, frp5 antibody, sFRP-5 antibody, sarp3 antibody, sfrp5 antibody, sfrp5-a antibody, sfrp5-b antibody, secreted frizzled related protein 5 antibody, secreted frizzled-related sequence protein 5 antibody, secreted frizzled-related protein 5 antibody, secreted frizzled-related protein 5 L homeolog antibody, secreted frizzled-related protein 5 S homeolog antibody, SFRP5 antibody, Sfrp5 antibody, sfrp5 antibody, sfrp5.L antibody, sfrp5.S antibody

Pathways: WNT Signaling