NEFL antibody (AA 281-396)
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- Target See all NEFL Antibodies
- NEFL (Neurofilament, Light Polypeptide (NEFL))
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Binding Specificity
- AA 281-396
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NEFL antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Purpose
- Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against NEFL. It has been selected for its ability to recognize NEFL in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Neurofilament, Light Polypeptide (NEFL) corresdonding to N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product NEFL Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- NEFL (Neurofilament, Light Polypeptide (NEFL))
- Alternative Name
- Neurofilament, Light Polypeptide (NEFL Products)
- Synonyms
- NEFL antibody, nefl antibody, zgc:136626 antibody, AI847934 antibody, CMT2E antibody, NF-L antibody, NF68 antibody, Nfl antibody, CMT1F antibody, NFL antibody, XNF-L antibody, neurofilament light antibody, neurofilament, light polypeptide antibody, neurofilament, light polypeptide b antibody, neurofilament, light L homeolog antibody, NEFL antibody, neflb antibody, Nefl antibody, nefl.L antibody
- Background
- CMT1F, CMT2E, NF-L, NF68, NFL, 68 kDa neurofilament protein, Neurofilament triplet L protein
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