REG3A antibody (AA 40-164) (Biotin)

Catalog No. ABIN1176323

Product Details anti-REG3A Antibody (Biotin)

Target: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Binding Specificity: AA 40-164

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REG3A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Sequence: MGHHHHHHSG SEF-C PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCN

Specificity: It has been selected for its ability to recognize REG3a in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant REG3a expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against REG3a conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant REG3a (Cys40~Asn164) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for REG3A

Target: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Alternative Name: Regenerating Islet Derived Protein 3 Alpha (REG3a) (REG3A Products)

Synonyms: HIP antibody, HIP/PAP antibody, INGAP antibody, PAP antibody, PAP-H antibody, PAP1 antibody, PBCGF antibody, REG-III antibody, REG3 antibody, AV063448 antibody, Pap2 antibody, PapII antibody, PTP antibody, REG3G antibody, regenerating family member 3 alpha antibody, regenerating islet-derived 3 alpha antibody, REG3A antibody, Reg3a antibody