FBLN3 antibody (AA 258-493) (Biotin)

Catalog No. ABIN1176446

Product Details anti-FBLN3 Antibody (Biotin)

Target: FBLN3 (Fibulin 3 (FBLN3))

Binding Specificity: AA 258-493

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBLN3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequence: MGHHHHHHSG SEF-CDA SNQCAQQCYN ILGSFICQCN QGYELSSDRL NCEDIDECRT SSYLCQYQCV NEPGKFSCMC PQGYQVVRSR TCQDINECET TNECREDEMC WNYHGGFRCY PRNPCQDPYI LTPENRCVCP VSNAMCRELP QSIVYKYMSI RSDRSVPSDI FQIQATTIYA NTINTFRIKS GNENGEFYLR QTSPVSAMLV LVKSLSGPRE HIVDLEMLTV SSIGTFRTSS VLRLTIIVGP FSF

Specificity: It has been selected for its ability to recognize FBLN3 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant FBLN3 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against FBLN3 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant FBLN3 (Cys258~Phe493) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for FBLN3

Target: FBLN3 (Fibulin 3 (FBLN3))

Abstract: FBLN3 Products

Synonyms: DHRD antibody, DRAD antibody, FBLN3 antibody, FBNL antibody, FIBL-3 antibody, MLVT antibody, MTLV antibody, S1-5 antibody, T16 antibody, EGF containing fibulin like extracellular matrix protein 1 antibody, EGF-containing fibulin-like extracellular matrix protein 1 antibody, epidermal growth factor-containing fibulin-like extracellular matrix protein 1 antibody, EFEMP1 antibody, Efemp1 antibody

Pathways: EGFR Signaling Pathway