L-Selectin antibody

Catalog No. ABIN1176858

Product Details anti-L-Selectin Antibody

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: C3H/eb mouse B lymphoma 38C-13

Clone: MEL-14

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-L-Selectin antibody (ABIN1176858)

Lanzavecchia, Sallusto: "Dynamics of T lymphocyte responses: intermediates, effectors, and memory cells." in: Science (New York, N.Y.), Vol. 290, Issue 5489, pp. 92-7, (2000) (PubMed).

Seibold, Seibold-Schmid, Cong, Shu, McCabe, Weaver, Elson: "Regional differences in L-selectin expression in murine intestinal lymphocytes." in: Gastroenterology, Vol. 114, Issue 5, pp. 965-74, (1998) (PubMed).

Cerwenka, Carter, Reome, Swain, Dutton: "In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 1, pp. 97-105, (1998) (PubMed).

Peschon, Slack, Reddy, Stocking, Sunnarborg, Lee, Russell, Castner, Johnson, Fitzner, Boyce, Nelson, Kozlosky, Wolfson, Rauch, Cerretti, Paxton, March, Black: "An essential role for ectodomain shedding in mammalian development." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1281-4, (1998) (PubMed).

Yang, Mizuno, Hellström, Chen: "B7-negative versus B7-positive P815 tumor: differential requirements for priming of an antitumor immune response in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 851-8, (1997) (PubMed).

Vestweber: "Ligand-specificity of the selectins." in: Journal of cellular biochemistry, Vol. 61, Issue 4, pp. 585-91, (1996) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Pizcueta, Luscinskas: "Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo." in: The American journal of pathology, Vol. 145, Issue 2, pp. 461-9, (1994) (PubMed).

Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, (1994) (PubMed).

Mobley, Dailey et al.: "Regulation of adhesion molecule expression by CD8 T cells in vivo. I. Differential regulation of gp90MEL-14 (LECAM-1), Pgp-1, LFA-1, and VLA-4 alpha during the differentiation of cytotoxic T ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 8, pp. 2348-56, (1992) (PubMed).

Iwabuchi, Ohgama, Ogasawara, Iwabuchi, Negishi, Good, Onoé: "Distribution of MEL-14+ cells in various lymphoid tissues." in: Immunobiology, Vol. 182, Issue 2, pp. 161-73, (1991) (PubMed).

Siegelman, Cheng, Weissman, Wakeland: "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." in: Cell, Vol. 61, Issue 4, pp. 611-22, (1990) (PubMed).

Jung, Gallatin, Weissman, Dailey: "Down-regulation of homing receptors after T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4110-7, (1989) (PubMed).

Kishimoto, Jutila, Berg, Butcher: "Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors." in: Science (New York, N.Y.), Vol. 245, Issue 4923, pp. 1238-41, (1989) (PubMed).

Shortman, Wilson, Van Ewijk, Scollay: "Phenotype and localization of thymocytes expressing the homing receptor-associated antigen MEL-14: arguments for the view that most mature thymocytes are located in the medulla." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 342-51, (1987) (PubMed).

Reichert, Jerabek, Gallatin, Butcher, Weissman: "Ontogeny of lymphocyte homing receptor expression in the mouse thymus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 10, pp. 3535-42, (1986) (PubMed).

Reichert, Weissman, Butcher: "Phenotypic analysis of thymocytes that express homing receptors for peripheral lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 10, pp. 3521-8, (1986) (PubMed).

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, (1983) (PubMed).

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L (L-selectin) (SELL Products)

Synonyms: CD62L antibody, LAM1 antibody, LECAM1 antibody, LEU8 antibody, LNHR antibody, LSEL antibody, LYAM1 antibody, PLNHR antibody, TQ1 antibody, A.11 antibody, L-selectin antibody, LECAM-1 antibody, SELE antibody, AI528707 antibody, Lnhr antibody, Ly-22 antibody, Ly-m22 antibody, Lyam-1 antibody, Lyam1 antibody, selectin L antibody, selectin, lymphocyte antibody, SELL antibody, Sell antibody

Target Type: Chemical

Background: The MEL-14 antibody reacts with CD62L (L-selectin), a 95 kDa (on neutrophils) or 74 kDa (on lymphocytes) receptor with lectin-like and Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from lymphocytes and neutrophils upon cell activation, metalloproteinases may mediate the release of CD62L ectodomains from the cell surface. The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to sialytaed oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that CD34, GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, Cat. No. 553863), may be ligands for CD62L. MEL-14 mAb blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and late stages of leukocyte rolling. This antibody is routinely tested by flow cytometric analysis.
Synonyms: L-selectin, LECAM-1, Ly-22