IFNGR1 antibody

Catalog No. ABIN1176897

Product Details anti-IFNGR1 Antibody

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFNGR1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of human IFN-gamma anti-viral activity. Neutralization may be measured with a encephalomyocarditis viral (EMCV) resistance assay using 600 pg/mL recombinant human IFN-gamma (Cat. No. 554617) with A549 indicator cells.
50% Neutralization (ND50) 0.2 - 2 µg/mL
> 90% Neutralization at 5 - 50 µg/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Human IFN-gammaRalpha

Clone: GIR

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IFNGR1 antibody (ABIN1176897)

Bach, Aguet, Schreiber: "The IFN gamma receptor: a paradigm for cytokine receptor signaling." in: Annual review of immunology, Vol. 15, pp. 563-91, (1997) (PubMed).

Peyman, Hammond: "Localization of IFN-gamma receptor in first trimester placenta to trophoblasts but lack of stimulation of HLA-DRA, -DRB, or invariant chain mRNA expression by IFN-gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2675-80, (1992) (PubMed).

Sheehan, Calderon, Schreiber: "Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4231-7, (1988) (PubMed).

Target Details for IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Alternative Name: CD119 (IFNGR1 Products)

Synonyms: CD119 antibody, IFNGR antibody, IFN-gammaR antibody, Ifgr antibody, Ifngr antibody, Nktar antibody, IFNGR1 antibody, MGC129098 antibody, ifngr1 antibody, MGC194858 antibody, MGC194873 antibody, zgc:194858 antibody, cd119 antibody, ifngr antibody, DKFZp469K1232 antibody, interferon gamma receptor 1 antibody, cytokine receptor family member B17 antibody, IFNGR1 antibody, Ifngr1 antibody, crfb17 antibody, ifngr1 antibody

Background: The GIR-208 antibody recognizes the extracellular region of CD119 which is also known as the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-gamma receptor (IFN-gammaRalpha). The functionally active-form of the human IFN-gamma receptor consists of two (or more) subunits, with IFN-gammaRalpha responsible for IFN-gamma binding and both the IFN-gammaRalpha and beta chains required for the transduction of biologic responses. The IFN-gamma receptor alpha chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. Binding of 125I-labeled GIR-208 antibody to IFN-gammaRalpha+ cells is reported to be specifically inhibited in the presence of excess IFN-gamma. GIR-208 does not cross react with IFN-gamma as tested by ELISA. The ability of this antibody to bind to IFN-gamma receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-gammaRalpha purified from human placenta. The GIR- 208 has been reported to block the binding of 125I-human IFN-gamma to IFN-gammaRalpha+ cells as well as purified, soluble human IFN-gammaRalpha. GIR-208 is a neutralizing antibody that has been shown to neutralize the anti-viral activity of IFN-gamma on WISH cells in a dose-dependent fashion.
Synonyms: IFN-gamma Receptor alpha chain, IFNgammaRalpha

Pathways: Interferon-gamma Pathway