IL-2 antibody

Catalog No. ABIN1176901

This Rat Monoclonal antibody specifically detects IL-2 in ELISA, Neut and ICS. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for IL-2 antibody (ABIN1176901)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Intracellular Staining (ICS)

Clone: MQ1-17H12

Product Details anti-IL-2 Antibody

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-2. Neutralization activity may be measured with a proliferation assay using 100 pg/mL recombinant human IL-2 (Cat. No. 554603) to stimulate CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-2 for 60 minutes).
50% Neutralization (ND50) at 0.1-1.5 µg/mL
>95% Neutralization at 3 - 15 myg/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Human IL-2 Recombinant Protein

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-2 antibody (ABIN1176901)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Andersson, Abrams, Björk, Funa, Litton, Agren, Andersson: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, (1995) (PubMed).

Fernandez, Andersson, Andersson, Troye-Blomberg: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." in: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, (1994) (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the MQ1-17H12 hybridoma was recombinant human IL-2. Unconjugated or purified forms of this antibody have been reported to be neutralizing for human IL-2 bioactivity.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation