B-Cell Linker antibody (N-Term) (Alexa Fluor 647)

Catalog No. ABIN1176912

Cited in 4+ publications. The Alexa Fluor 647-conjugated Mouse Monoclonal anti-B-Cell Linker antibody (Clone 2B11) (ABIN1176912) specifically detects B-Cell Linker in ICS. The antibody is reactive with Human and Mouse samples.

Quick Overview for B-Cell Linker antibody (N-Term) (Alexa Fluor 647) (ABIN1176912)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This B-Cell Linker antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: 2B11

Product Details anti-B-Cell Linker Antibody (Alexa Fluor 647)

Binding Specificity: N-Term

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human N-terminal BLNK Recombinant Protein

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-B-Cell Linker antibody (Alexa Fluor 647) (ABIN1176912)

Taguchi, Kiyokawa, Takenouch, Matsui, Tang, Nakajima, Suzuki, Shiozawa, Saito, Katagiri, Takahashi, Karasuyama, Matsuo, Okita, Fujimoto: "Deficiency of BLNK hampers PLC-gamma2 phosphorylation and Ca2+ influx induced by the pre-B-cell receptor in human pre-B cells." in: Immunology, Vol. 112, Issue 4, pp. 575-82, (2004) (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Li, Martin, Oliver, Kearney, Carter: "Antigen receptor proximal signaling in splenic B-2 cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3122-9, (2001) (PubMed).

Minegishi, Rohrer, Coustan-Smith, Lederman, Pappu, Campana, Chan, Conley: "An essential role for BLNK in human B cell development." in: Science (New York, N.Y.), Vol. 286, Issue 5446, pp. 1954-7, (1999) (PubMed).

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The 2B11 monoclonal antibody recognizes BLNK, regardless of phosphorylation status. A fusion protein representing amino acids 4-205 of human BLNK was used as the immunogen. BLNK is expressed as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE that represent alternatively spliced forms of human BLNK.
Synonyms: SLP-65, BASH, BCA

Pathways: BCR Signaling