PDGFRA antibody

Catalog No. ABIN1176995

Product Details anti-PDGFRA Antibody

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse PDGF Receptor alpha chain

Clone: APA5

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-PDGFRA antibody (ABIN1176995)

Fruttiger, Calver, Krüger, Mudhar, Michalovich, Takakura, Nishikawa, Richardson: "PDGF mediates a neuron-astrocyte interaction in the developing retina." in: Neuron, Vol. 17, Issue 6, pp. 1117-31, (1997) (PubMed).

Takakura, Yoshida, Kunisada, Nishikawa, Nishikawa: "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 770-7, (1996) (PubMed).

Heldin: "Structural and functional studies on platelet-derived growth factor." in: The EMBO journal, Vol. 11, Issue 12, pp. 4251-9, (1992) (PubMed).

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: CD140A (PDGFRA Products)

Background: The APA5 antibody monoclonal antibody specifically binds to the Platelet-Derived Growth Factor (PDGF) Receptor alpha chain (CD140a, PDGFR-a), a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult and on several other cell types during embryonic development, but not on hematopoietic cells. PDGFR-a binds to PDGF A and B chains, in contrast to PDGFR-b, that binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The APA5 antibody has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
Synonyms: Pdgfra, Pdgfr2, PDGF-R alpha, Platelet derived growth factor receptor alpha

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling