EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr36) (Alexa Fluor 647)

Details for Product anti-EIF4EBP1 Antibody No. ABIN1177022
Antigen
  • 4E-BP1
  • 4EBP1
  • BP-1
  • PHAS-I
  • 4e-bp1
  • AA959816
  • 153432_at
  • 43-BP
  • 4E-BP
  • 4EBP
  • 4e-bp
  • 4eBP
  • 4ebp
  • BcDNA.HL08053
  • BcDNA:HL08053
  • CG8846
  • CT8705
  • Dm 4E-BP
  • Dmel\\CG8846
  • Phas1
  • Thor/EIF4EBP
  • anon-WO0172774.158
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4e-bp
  • d4eBP
  • dthor
  • eIF4E-BP
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • pp20
  • thor
  • EIF4EBP1
  • fc04h09
  • wu:fc04h09
  • zgc:64137
  • eukaryotic translation initiation factor 4E binding protein 1
  • CG8846 gene product from transcript CG8846-RA
  • EIF4EBP1
  • Eif4ebp1
  • Thor
  • eif4ebp1
Epitope
pThr36
58
36
35
31
28
24
24
17
16
16
15
15
15
13
12
11
11
9
6
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
511
329
278
15
13
13
7
7
3
3
3
2
1
1
1
1
1
Host
Mouse
489
63
9
Clonality (Clone)
Monoclonal ()
Conjugate
This EIF4EBP1 antibody is conjugated to Alexa Fluor 647
32
23
13
12
12
12
12
12
12
12
8
7
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
355
262
197
81
66
37
27
17
17
13
7
6
4
2
2
1
Options
Brand BD Phosflow™
Immunogen Phosphorylated Human 4EBP1 (pT45) Peptide
Clone M31-16
Isotype IgG1, kappa
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others EIF4EBP1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name 4EBP1 (EIF4EBP1 Antibody Abstract)
Background The eukaryotic translation initiation factor 4E-Binding Protein 1 (4EBP1) is a phosphorylated heat- and acid-stable protein (PHAS-I or PHAS-1), and it is regulated by insulin. It is a member of the eIF4E-Binding Protein Family, which also includes the proteins 4EBP2 and 4EBP3. 4EBP1 binds with eukaryotic translation Initiation Factor 4E (eIF4E), which prevents its assembly into the eIF4E complex and inhibits cap-dependent translation. When 4EBP1 is phosphorylated, this binding is disrupted, allowing cap-dependent translation to be activated. Phosphorylation of 4EBP1 is required for protein synthesis, and it mediates the regulation of protein translation by stimuli that signal through the phosphoinositide 3 (PI3) kinase pathway. We found that threonines 36 and 45 (T36/T45) are phosphorylated in resting human peripheral blood mononuclear cells. PI3 kinase inhibitors, such as LY294002 down-regulate the phosphorylation level of 4EBP1 (pT36/pT45). The M31-16 monoclonal antibody recognizes the phosphorylated T36 and T45 of activated human 4EBP1. The orthologous phosphorylation sites in mouse and rat 4EBP1 are T35 and T44.
Synonyms: 4E-BP1. EIF4EBP1, P/OKCL.6, PHAS-I, PHAS-1
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product cited in: Hay, Sonenberg: "Upstream and downstream of mTOR." in: Genes & development, Vol. 18, Issue 16, pp. 1926-45, 2004 (PubMed).

Gingras, Raught, Gygi, Niedzwiecka, Miron, Burley, Polakiewicz, Wyslouch-Cieszynska, Aebersold, Sonenberg: "Hierarchical phosphorylation of the translation inhibitor 4E-BP1." in: Genes & development, Vol. 15, Issue 21, pp. 2852-64, 2001 (PubMed).

Did you look for something else?