Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

B-Cell Linker antibody (pTyr84) (Alexa Fluor 647)

BLNK Reactivity: Human, Mouse ICS Host: Mouse Monoclonal J117-1278 Alexa Fluor 647
Catalog No. ABIN1177037
  • Target See all B-Cell Linker (BLNK) Antibodies
    B-Cell Linker (BLNK)
    Binding Specificity
    • 24
    • 22
    • 17
    • 15
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr84
    Reactivity
    • 103
    • 68
    • 38
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 113
    • 8
    • 1
    Mouse
    Clonality
    • 108
    • 14
    Monoclonal
    Conjugate
    • 52
    • 8
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    This B-Cell Linker antibody is conjugated to Alexa Fluor 647
    Application
    • 109
    • 52
    • 36
    • 26
    • 26
    • 19
    • 18
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Human BLNK Peptide
    Clone
    J117-1278
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product BLNK Primary Antibody
  • Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
  • Pighi, Gu, Dalai, Barbi, Parolini, Bertolaso, Pedron, Parisi, Ren, Cecconi, Chilosi, Menestrina, Zamò: "Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling." in: Cellular oncology (Dordrecht), Vol. 34, Issue 2, pp. 141-53, (2011) (PubMed).

    Espeli, Mancini, Breton, Poirier, Schiff: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).

  • Target
    B-Cell Linker (BLNK)
    Alternative Name
    BLNK (BLNK Products)
    Synonyms
    BLNK antibody, blnk antibody, MGC147045 antibody, BASH antibody, Bca antibody, Ly-57 antibody, Ly57 antibody, Lyw-57 antibody, SLP-65 antibody, AGM4 antibody, BLNK-S antibody, LY57 antibody, SLP65 antibody, bca antibody, B-cell linker antibody, B cell linker antibody, BLNK antibody, blnk antibody, Blnk antibody
    Background
    B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.
    Pathways
    BCR Signaling
You are here:
Support