B-Cell Linker antibody (pTyr84) (Alexa Fluor 488)

Catalog No. ABIN1177038

Cited in 1 publication. The Alexa Fluor 488-conjugated Mouse Monoclonal anti-B-Cell Linker antibody (Clone J117-1278) (ABIN1177038) specifically detects B-Cell Linker in ICS. The antibody is reactive with Human and Mouse samples.

Quick Overview for B-Cell Linker antibody (pTyr84) (Alexa Fluor 488) (ABIN1177038)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This B-Cell Linker antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: J117-1278

Product Details anti-B-Cell Linker Antibody (Alexa Fluor 488)

Binding Specificity: pTyr84

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human BLNK Peptide

Isotype: IgG2b kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-B-Cell Linker antibody (Alexa Fluor 488) (ABIN1177038)

Chiu, Dalton, Ishiai, Kurosaki, Chan: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." in: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, (2002) (PubMed).

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Pathways: BCR Signaling