JNK antibody (pThr183) (Alexa Fluor 647)

Catalog No. ABIN1177076

The Mouse Monoclonal anti-JNK antibody has been validated for ICS. It is suitable to detect JNK in samples from Human and Mouse. There are 4+ publications available.

Quick Overview for JNK antibody (pThr183) (Alexa Fluor 647) (ABIN1177076)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This JNK antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: N9-66

Product Details anti-JNK Antibody (Alexa Fluor 647)

Binding Specificity: pThr183

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human JNK Peptide

Isotype: IgG1 kappa

Application Details

Sample Volume: 5 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-JNK antibody (Alexa Fluor 647) (ABIN1177076)

Huang, Shi, Chi: "Regulation of JNK and p38 MAPK in the immune system: signal integration, propagation and termination." in: Cytokine, Vol. 48, Issue 3, pp. 161-9, (2009) (PubMed).

Wagner, Nebreda: "Signal integration by JNK and p38 MAPK pathways in cancer development." in: Nature reviews. Cancer, Vol. 9, Issue 8, pp. 537-49, (2009) (PubMed).

Fleming, Armstrong, Morrice, Paterson, Goedert, Cohen: "Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7." in: The Biochemical journal, Vol. 352 Pt 1, Issue 3, pp. 145-54, (2001) (PubMed).

Kyriakis, Avruch: "Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation." in: Physiological reviews, Vol. 81, Issue 2, pp. 807-69, (2001) (PubMed).

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK

Background: The N9-66 monoclonal antibody specifically binds to JNK1 and JNK2 phosphorylated at the pT183/pY185 sites. c-Jun NH2-terminal Kinases (JNKs), also called Stress Activated Protein Kinases (SAPKs), are mitogen-activated protein kinases (MAPKs) with observed molecular weights of ~46 kDa (JNK1) and ~54 kDa (JNK2). Along with the p38 and ERK families, JNK represents one of three major classes of MAPKs. Complete activation of JNK requires the phosphorylation of both Thr183 and Tyr185 that are located in a Thr-X-Tyr motif. Phosphorylation of these residues is carried out by MKK4 and MKK7 that are phosphorylated and activated by MEKKs and MLKs in response to stress signals delivered through small GTPases of the Rho family. Once activated, JNK can translocate into the nucleus and regulate the expression of genes through phosphorylation of c-Jun, ATF-2, and other transcription factors. JNK plays a role in signal transduction in response to cytokines and various forms of environmental stress, such as endotoxins, UV irradiation, heat, and hyperosmolarity. JNK is critical to the regulation of cell growth, apoptosis, and the cellular response to stress, making it an important factor in tumorigenesis and adaptive immunity. During antibody development, the N9-66 monoclonal antibody was found to detect phosphorylated JNK1/2 by Western blot analysis of cellular lysates and by immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells. This antibody crossreacts with phosphorylated JNK1/2 expressed by mouse cells, as tested by Western blot analysis and flow cytometry.
Synonyms: MAPK8, MAPK9, SAPK1, SAPK, PRKM8, PRKM9

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins