MAPKAP Kinase 2 antibody (pThr334)

Catalog No. ABIN1177086

This anti-MAPKAP Kinase 2 antibody is a Mouse Monoclonal antibody detecting MAPKAP Kinase 2 in WB and ICS. Suitable for Human and Mouse. This Primary Antibody has been cited in 8+ publications.

Quick Overview for MAPKAP Kinase 2 antibody (pThr334) (ABIN1177086)

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPKAP Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB), Intracellular Staining (ICS)

Clone: P24-694

Product Details anti-MAPKAP Kinase 2 Antibody

Binding Specificity: pThr334

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human MAPKAPK-2 Peptide

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MAPKAP Kinase 2 antibody (ABIN1177086)

Manke, Nguyen, Lim, Stewart, Elia, Yaffe: "MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation." in: Molecular cell, Vol. 17, Issue 1, pp. 37-48, (2005) (PubMed).

Wang, Xu, Wang, Young, Gaestel, Feuerstein: "Mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 deficiency protects brain from ischemic injury in mice." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43968-72, (2002) (PubMed).

Heidenreich, Neininger, Schratt, Zinck, Cahill, Engel, Kotlyarov, Kraft, Kostka, Gaestel, Nordheim: "MAPKAP kinase 2 phosphorylates serum response factor in vitro and in vivo." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14434-43, (1999) (PubMed).

Engel, Kotlyarov, Gaestel: "Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation." in: The EMBO journal, Vol. 17, Issue 12, pp. 3363-71, (1998) (PubMed).

Krump, Sanghera, Pelech, Furuya, Grinstein: "Chemotactic peptide N-formyl-met-leu-phe activation of p38 mitogen-activated protein kinase (MAPK) and MAPK-activated protein kinase-2 in human neutrophils." in: The Journal of biological chemistry, Vol. 272, Issue 2, pp. 937-44, (1997) (PubMed).

Ben-Levy, Leighton, Doza, Attwood, Morrice, Marshall, Cohen: "Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2." in: The EMBO journal, Vol. 14, Issue 23, pp. 5920-30, (1996) (PubMed).

Clifton, Young, Cohen: "A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress." in: FEBS letters, Vol. 392, Issue 3, pp. 209-14, (1996) (PubMed).

Rouse, Cohen, Trigon, Morange, Alonso-Llamazares, Zamanillo, Hunt, Nebreda: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, (1994) (PubMed).

Target Details for MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Alternative Name: MAPKAPK-2

Background: The P24-694 monoclonal antibody specifically binds to the phosphorylated T334 site (pT334) of MAPKAPK-2. MAPKAPK-2 is a serine/threonine protein kinase. This ~49 kDa member of the MAPKAPK family of protein kinases is also known as mitogen-activated protein kinase-activated protein kinase 2. MAPKAPK-2 is phosphorylated and activated by p38 MAP kinase in response to stress, cytokines and chemokines. MAPKAPK-2 is phosphorylated on multiple sites including Thr222, Ser272 and Thr334. Phosphorylation of any two of these three amino acid residues seems to be required for the activation of this kinase that serves multiple cellular functions. Phosphorylation of Thr334 was reported to be essential for nuclear export of the heterodimer formed between p38 MAPK and MAPKAPK-2. Mice deficient in MAPKAPK-2 have been shown to be protected from ischemic injury. MAPKAPK-2 is also reported to serve as a cell cycle checkpoint kinase in response to UV irradiation. The heat shock protein, HSP27 was shown to be one of the major substrates of MAPK and MAPKAPK-2.
Synonyms: MAP kinase-activated protein kinase 2, MAPKAP kinase 2, MK2, MAPK2 Entrez Gene ID: 9261, 17164

Molecular Weight: ~49 kDa

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades