Smad1 , Smad8 (AA 456-465), (pSer463), (pSer465) antibody (PE)

Catalog No. ABIN1177177

This Rat Monoclonal antibody specifically detects Smad1 , Smad8 in ICS. It exhibits reactivity toward Human and Mouse. It has been mentioned in 7+ publications

Quick Overview for Smad1 , Smad8 (AA 456-465), (pSer463), (pSer465) antibody (PE) (ABIN1177177)

Target: Smad1 , Smad8

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: PE

Application: Intracellular Staining (ICS)

Clone: N6-1233

Product Details

Binding Specificity: AA 456-465, pSer463, pSer465

Brand: BD Phosflow™

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography

Immunogen: Phosphorylated Human Smad1 aa 456-465 Peptide

Isotype: IgG2a kappa

Application Details

Sample Volume: 5 μL

Protocol: In human PBMC, overnight serum starvation was found to be necessary for detection of a BMP-6-induced increase in Smad1/8 phosphorylation. Serum starvation for 1 hour following PBMC isolation was not sufficient to reduce basal levels of Smad1 (pS463/pS465)/Smad8(pS465/pS467). Some donor variation was observed in the reduction of basal phosphorylation by overnight serum starvation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References

Kersten, Dosen, Myklebust, Sivertsen, Hystad, Smeland, Rian: "BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3." in: Experimental hematology, Vol. 34, Issue 1, pp. 72-81, (2006) (PubMed).

Kersten, Sivertsen, Hystad, Forfang, Smeland, Myklebust: "BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1." in: BMC immunology, Vol. 6, pp. 9, (2005) (PubMed).

Whitman: "Smads and early developmental signaling by the TGFbeta superfamily." in: Genes & development, Vol. 12, Issue 16, pp. 2445-62, (1998) (PubMed).

Kretzschmar, Doody, Massagué: "Opposing BMP and EGF signalling pathways converge on the TGF-beta family mediator Smad1." in: Nature, Vol. 389, Issue 6651, pp. 618-22, (1997) (PubMed).

Kretzschmar, Liu, Hata, Doody, Massagué: "The TGF-beta family mediator Smad1 is phosphorylated directly and activated functionally by the BMP receptor kinase." in: Genes & development, Vol. 11, Issue 8, pp. 984-95, (1997) (PubMed).

Hogan: "Bone morphogenetic proteins: multifunctional regulators of vertebrate development." in: Genes & development, Vol. 10, Issue 13, pp. 1580-94, (1996) (PubMed).

Hoodless, Haerry, Abdollah, Stapleton, OConnor, Attisano, Wrana: "MADR1, a MAD-related protein that functions in BMP2 signaling pathways." in: Cell, Vol. 85, Issue 4, pp. 489-500, (1996) (PubMed).

Target Details

Target: Smad1 , Smad8

Background: The N6-1233 monoclonal antibody specifically binds to the Smad1 protein phosphorylated at the Ser463/465 sites and the Smad8 protein phosphorylated at the Ser465/467 sites. Smad1 and Smad8 are ~60 kDa proteins and are members of the Smad superfamily. The Smad family members are divided into three subfamilies: receptor regulated Smads or R-Smads, including Smads1, 2, 3, 5, and 8, common partner Smad, or Co-Smad, including Smad4, and inhibitory Smads, or I-Smad, including Smads 6 and 7. Activation of Transforming Growth Factor-beta (TGF-beta) superfamily serine/threonine kinase receptors, such as TGF-beta and Bone Morphogenic Protein (BMP) receptors, leads to the phosphorylation of R-Smads at several sites. It has been shown that Ser463 and Ser465 of Smad1 are phosphorylated by BMP receptors. In B cells and pre-B cells, BMP-6 has been shown to induce Smad1/5/8 phosphorylation and inhibit cell proliferation. Phosphorylated R-Smads form complexes with Co-Smad and translocate into the nucleus to regulate transcription affecting a wide range of critical processes including cell-fate determination, proliferation, morphogenesis, differentiation and apoptosis. The inhibitory Smads inhibit this pathway through two potential mechanisms: either by preventing R-Smads from binding to their corresponding receptors and/or by competing with Smad4, the Co-Smad, from binding to R-Smads. This antibody may crossreact with Smad5 pS463/pS465 based on sequence homology.
Synonyms: Smad1,Smad8/Smad9, MADH1, MADH9