Integrin alpha 1 antibody

Catalog No. ABIN1177273

This Armenian Hamster Monoclonal antibody specifically detects Integrin alpha 1 in WB, IP, FACS, IHC (fro), BR and IHC (f). It exhibits reactivity toward Mouse and Rat. It has been mentioned in 3+ publications

Quick Overview for Integrin alpha 1 antibody (ABIN1177273)

Target: Integrin alpha 1 (ITGA1)

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This Integrin alpha 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: Ha31-8

Product Details anti-Integrin alpha 1 Antibody

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Rat Emulsified Lewis Rat Glomeruli Cells

Isotype: IgG2 lambda

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-Integrin alpha 1 antibody (ABIN1177273)

Mendrick, Kelly, duMont, Sandstrom: "Glomerular epithelial and mesangial cells differentially modulate the binding specificities of VLA-1 and VLA-2." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 72, Issue 3, pp. 367-75, (1995) (PubMed).

Miyake, Sakurai, Okumura, Yagita: "Identification of collagen and laminin receptor integrins on murine T lymphocytes." in: European journal of immunology, Vol. 24, Issue 9, pp. 2000-5, (1994) (PubMed).

Hemler: "VLA proteins in the integrin family: structures, functions, and their role on leukocytes." in: Annual review of immunology, Vol. 8, pp. 365-400, (1990) (PubMed).

Target Details for Integrin alpha 1

Target: Integrin alpha 1 (ITGA1)

Alternative Name: CD49a

Background: The Ha31/8 antibody reacts with the 180-kDa integrin alpha1 chain (CD49a), which is a transmembrane glycoprotein that non-covalently associates with the integrin beta1 subunit (CD29) to form the alpha1beta1 (complex known as VLA-1). VLA-1 has been reported to be expressed on activated T cells, monocytes, smooth muscle cells, and endothelial cells. It is a receptor for collagen and laminin. The Ha31/8 monoclonal antibody is specific for both rat and mouse CD49a. It has been reported that Ha31/8 antibody can block VLA-1-mediated binding of rat cells to collagen.
Synonyms: Integrin alpha1 chain

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex