Interferon gamma antibody

Catalog No. ABIN1177289

There are 4+ publications for this product available. The Armenian Hamster Monoclonal anti-Interferon gamma antibody is suitable to detect Interferon gamma in samples from Mouse. It has been validated for ELISA and Neut.

Quick Overview for Interferon gamma antibody (ABIN1177289)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: ELISA, Neutralization (Neut)

Clone: H22

Product Details anti-Interferon gamma Antibody

Characteristics: Neutralization Activity: 50% Neutralization (ND50) at 1-150 ng/mL
>95% Neutralization at 60-500 ng/ml

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant Mouse IFN-gamma

Isotype: IgG1 kappa

Application Details

Application Notes: In vitro neutralization: The NA/LE format of the H22 antibody (Cat. No. 557530) is useful for the in vitro neutralization of mouse IFN-gamma bioactivity. Assay Type: EMC viral resistance assay Using 1.0 ng/ml recombinant mouse IFN-gamma (Cat. No. 554587) and preincubation of antibody and ligand = 60 minutes Indicator cell: L929 at 2 x10^5 cells/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-Interferon gamma antibody (ABIN1177289)

Leiby, Fortier, Crawford, Schreiber, Nacy: "In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies." in: Infection and immunity, Vol. 60, Issue 1, pp. 84-9, (1992) (PubMed).

Bancroft, Schreiber, Bosma, Bosma, Unanue: "A T cell-independent mechanism of macrophage activation by interferon-gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 4, pp. 1104-7, (1987) (PubMed).

Buchmeier, Schreiber: "Requirement of endogenous interferon-gamma production for resolution of Listeria monocytogenes infection." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 21, pp. 7404-8, (1985) (PubMed).

Green, Yeh, Overall: "Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Background: The H22 antibody reacts with mouse interferon-gamma (IFN-gamma , but not mouse IFN-alpha , IFN-beta , IL-6, or human IFN-gamma. The H22 antibody was reported to inhibit the in vitro anti-viral, the class II MHC antigen-inducing and the macrophage-activating factor (MAF) activities of mouse IFN-gamma. Moreover, the IFN-gamma neutralizing capacity of has been reported in several in vivo model systems. The specific binding of I[125] -labeled recombinant mouse IFN-gamma to IFN-gamma receptor-positive cells is blocked by H22. Although the H22 antibody is reported to react with rat IFN-gamma , its reactivity with IFN-gamma from other species has not been tested.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy