ITGA4 antibody

Catalog No. ABIN1177343

The Mouse Monoclonal anti-ITGA4 antibody has been validated for FACS. It is suitable to detect ITGA4 in samples from Human, Cow, Dog, Horse, Cat, Sheep, Cynomolgus, Rhesus Monkey and Baboon. There is 1 publication available.

Quick Overview for ITGA4 antibody (ABIN1177343)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Cow, Dog, Horse, Cat, Sheep, Cynomolgus, Rhesus Monkey, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 9F10

Product Details anti-ITGA4 Antibody

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-ITGA4 antibody (ABIN1177343)

Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: The 9F10 monoclonal antibody specifically reacts with the integrin alpha4 chain, that is expressed as a heterodimer with either of two beta integrin subunits, beta1 (CD29) or beta7. The alpha4beta1 integrin (VLA-4) is expressed on lymphocytes, monocytes, thymocytes, NK cells, and several B- and T-cell lines, and mediates binding to VCAM-1 (CD106) and the CS-1 region of fibronectin. The alpha4beta7 integrin has a similar tissue distribution, except it is found on only a small subpopulation of thymocytes. Integrin alpha4beta7 also binds fibronectin and VCAM-1, and has been shown in the mouse to preferentially bind the mucosal vascular addressin molecule, MAdCAM-1. This antibody is useful for studies of the expression by and function of cells that express alpha4 chain-containing integrins.
Synonyms: Integrin alpha4 chain, Integrin alpha 4, ITGA4, IA4, alpha 4 subunit of VLA-4

Pathways: Integrin Complex