ITGA4 antibody

Catalog No. ABIN1177343

There is 1 publication for this product available. The Mouse Monoclonal anti-ITGA4 antibody is suitable to detect ITGA4 in samples from Human, Cow, Dog, Horse, Cat, Sheep, Cynomolgus, Rhesus Monkey and Baboon. It has been validated for FACS.

Quick Overview for ITGA4 antibody (ABIN1177343)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Cow, Dog, Horse, Cat, Sheep, Cynomolgus, Rhesus Monkey, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 9F10

Product Details anti-ITGA4 Antibody

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-ITGA4 antibody (ABIN1177343)

Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: The 9F10 monoclonal antibody specifically reacts with the integrin alpha4 chain, that is expressed as a heterodimer with either of two beta integrin subunits, beta1 (CD29) or beta7. The alpha4beta1 integrin (VLA-4) is expressed on lymphocytes, monocytes, thymocytes, NK cells, and several B- and T-cell lines, and mediates binding to VCAM-1 (CD106) and the CS-1 region of fibronectin. The alpha4beta7 integrin has a similar tissue distribution, except it is found on only a small subpopulation of thymocytes. Integrin alpha4beta7 also binds fibronectin and VCAM-1, and has been shown in the mouse to preferentially bind the mucosal vascular addressin molecule, MAdCAM-1. This antibody is useful for studies of the expression by and function of cells that express alpha4 chain-containing integrins.
Synonyms: Integrin alpha4 chain, Integrin alpha 4, ITGA4, IA4, alpha 4 subunit of VLA-4

Pathways: Integrin Complex